OPTIMIZATION OF SOLVENTS FOR THE EXTRACTION AND METHODS FOR QUANTIFICATION OF VITAMIN E FROM SOYMEAL

  • Navita Bansal
  • Rama Prashat G.
  • Vanchinathan S.
  • Vinutha T.
  • Shelly Praveen
Keywords: Solvent, Soymeal, Vitamin, Quantification

Abstract

Extraction of vitamin E from soymeal (the product obtained after oil extraction) was carried out using six different solvents viz., Methanol, n-hexane, 2-Methyltetrahydrofuran, Methanol:n-hexane:2-MeTHF(80:10:10),    0.1 N H2SO4 followed by ethanol extraction and Petroleum ether: ethanol (2:1.6) and two spectrophotometric methods: Bathophenanthroline and Ammonium Phospho Molybdate (APM) methods. The results showed significantly higher yield of Vit-E (7.5 gm/100gm) with a recovery percentage of 83.3% when extracted using 0.1N sulfuric acid treatment followed by ethanol. These values were further validated by HPLC quantification.  Whereas bathophenanthroline method was found more suitable method to quantify Vit-E extracted from oil samples using non polar solvents (data not shown). Thus our study helps to select the suitable solvents for the extraction of Vit-E and the selection of solvents depends on the type of the sample (oil or powder form). Further our study also revealed that, methods for the estimation of Vit-E should be carefully selected as the extraction of Vit-E is related to the type of solvents (polar and non-polar) used.

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Author Biographies

Navita Bansal

Division of Biochemistry, IARI, New Delhi-110012

Rama Prashat G.

Division of Genetics, IARI, New Delhi-110012

Vanchinathan S.

Division of Genetics, IARI, New Delhi-110012

Vinutha T.

Division of Biochemistry, IARI, New Delhi-110012

Shelly Praveen

Division of Biochemistry, IARI, New Delhi-110012

Published
2020-06-30
How to Cite
Bansal, N., G., R., S., V., T., V., & Praveen, S. (2020, June 30). OPTIMIZATION OF SOLVENTS FOR THE EXTRACTION AND METHODS FOR QUANTIFICATION OF VITAMIN E FROM SOYMEAL. Innovative Farming, 5(2), 60-66. Retrieved from http://innovativefarming.in/index.php/innofarm/article/view/340
Section
Articles